Grassland ecosystems face imminent threat from a variety of sources, including invasive species. Chief among these invasive species is spotted knapweed (Centaurea maculosa). We devised a study to examine the effectiveness of fire as a control agent of spotted knapweed and the allelopathic chemical it produces, (±)-catechin. We conducted our experiment in part of a restored prairie ecosystem at Pierce Cedar Creek Institute in Barry County, Michigan in summer 2016. Our experiment consisted of burning established 1m² plots at high and low temperatures across spring and summer seasons, then planting six native prairie plant species as a bioassay for the presence of (±)-catechin. Overall, spotted knapweed removal was more effective with summer burns than with spring burns and planted native species established slightly better in burned plots than unburned plots. Results suggest that prescribed burning may be an effective tool for removing spotted knapweed and may aid native species establishment.