Tributary streams are primarily fed and sustained by external water sources and thus are extremely susceptible to anthropogenic changes. Because these streams are continually altered, organisms within these streams may exhibit increased genetic variation. Populations with high levels of genetic diversity are more resilient and resilient to anthropogenic disturbances and alterations. When populations become too small or homogenize, it is possible to fix deleterious alleles and populations may go extinct. While many studies show that genetic variation exists in populations that are separated by physical barriers, little is known about sympatric populations. Species of caddisflies within the family Hydropsychidae are indicators of environmental quality and have high mobility which allows a potential increase in interbreeding. Our goal is to understand the genetic variation between Hydropsychidae populations within isolated tributary streams and to further investigate baseline macroinvertebrate populations as an intermediate step in a long-term restoration project.